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Effect of inhibition of the MEK pathway on EZH2 expressionWe proceeded to analyze whether inhibition of the MEK pathway would affect EZH2 overexpression. First, The cytotoxic effect on the MEK inhibitor (U0126) On MDA MB 231 cells was applied by analyzing the cell growth curve. Knockdown within Elk 1, The functional transcribing factor present in the downstream of the MEK pathway, By simple interfering RNA (siRNA) Transfection caused a decrease in level of EZH2 mRNA expression that was just like the decrease caused by MEK inhibitor treatment (sleek figure 2b).
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