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Based on primary sequence similarity, The G proteins can be considered into four major sub families, i/o, s, Q/11 and simply 12/13, And each couples with a different group of GPCRs and regulates different signalling pathways1. The three dimensional structures of G have a conserved protein fold, Which comprises a GTPase domain and a helical domain (additional Fig. 1a). The GTPase domain holds five helices (1 5) adjacent six sheets (1 6), And is homologous to the Ras like small GTPase protein. The helical domain covers six helices (A farrenheit), And is for this GTPase domain by two linker regions. The bound guanine nucleotide forms interactions with residues from both of the domains, And resides in a cleft regarding the two domains (extra Fig. 1b,h). The GTPase domain contains three open loops, Named goes 1, 2 and then 3, Where decent structural changes occur between the G GTP and G GDP structures. These regions initiate binding to G, The effector necessary protein, and in addition RGS.