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A functional, Polytene discoloration with S112 GlcNAc (fresh) additionally DAPI (glowing blue) collectively with (reddish, highest), (teal, inner) perhaps (ured, foot). B ChIP seq research into the H2B S112 GlcNAc and K120 monoubiquitination. The distributions of H2B S112 GlcNAc experienced averaged near TSS (the best) and thus TTS (ground level) (f). The average profiles of H2B S112 GlcNAc near TSS were calculated based on the associated gene activities (f). Venn blueprints showing overlap of the peaks (def, positioned), as genes (defense, average) And the promoter (k, appropriately) Harbouring the modifications. The ChIP seq profile enfolding the GSK3B gene (a). Arrowhead, state of qPCR primer. f, ChIP consent in the GSK3B promoter. After Glc exhaustion, The take care of HeLa cells (schokofarbene bar) and OGT knockdown cells (green bar) Were rejuvenated with Glc for 24 Then, The cells were pass through ChIP with the indicated antibody and qPCR analysis. (n = 3).
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