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An actual, marketing of Ct values for the RT qPCR+ samples tested during the ZiBRA journey in Brazil (N=181 examples; average Ct=35.96). w, Distribution of the temporal lag between the date of onset of clinical symptoms and the date of sample collection of RT qPCR+ samples (average lag, 2 days). Red dashed lines characterize the median of the distributions. g, approval of sequencing approaches. A phylogeny of the ZIKV Asian genotype roughly using PhyML38 is shown. The expanded clade highlighted in blue offers the WHO reference ZIKV sequence20 (Accession KX369547), that had been generated using Illumina MiSeq. Sequences made using MinION chemistries R9.4 2D, R9.4 1D, R9 1D, R9 2D since R7.3 2D contain no nucleotide disparities and hence were also placed in this clade. Scale bars represent expected nucleotide alternatives per site (s/s).
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