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Top of pageResultsMaterials and methods Results Discussion references Acknowledgements Figures and TablesTelomerase and cell cycle regulator in primary human OSE and transduced cellsAll transduced cells showed telomerase activity in a TRAP assay at passage 7 (Data not viewable). In primary person’s OSE cells, P16 protein levels were quite high since passage 4, And numbers of p53 and p21 proteins increased through passage 4 (sum 1). It could be supported by nearly everywhere blockage of Rb pathway enhances p53 pathway via induction of p14ARF (Bates et al, 1998). Our data were in keeping with previous studies that immortalised cells by inactivating Rb pathway introducing E7 highly expressed p53, p21, combined with p16 (instill and Galloway, 1996; Kiyono et al, 1998; Jarrard et al, 1999; Mori et ing, 2005). therefore, They were defined as immortalised cell lines. aside from that, These five cell lines are now growing with a population doubling number above 200. in addition, In the anchorage dependent spreading assay, Cell proliferation of HOSE1s cells was certainly higher than that of primary human OSE cells (k (calculate 2).