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Black circles denote Fluorescein GABA RRRRRRRR Photo cleavable linker, which might be cleaved by UV. White bags denote Fluorescein GABA RRRRRRRR 11 aminoundecanoic acid, Which can’t cleaved by UV (destructive control). Peptide concentration was determined by measuring the length fluorescence. The standard curve of the purified Fluorescein GABA RRRRRRRR was used to determine the concentration of the cleaved peptide.Fluorescein GABA R8 on 11 aminoundecanoic acid was not cleaved from the arrays within 3h of irradiation because this linker did not have a photo cleavable site (Fig.
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