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EIF5A undergoes tyrosine sulfationTo identify the type and amino acid site of this eIF5A a static correction, We analyzed purified produced and cytosolic re eIF5A via nanoLC LTQ orbitrap MS. The molecular weight of the peptide complimenting to residues 68 85 was 80Da higher than expected (Fig. 2a h). Candidate differences to explain this 80Da increase included phosphorylation and sulfation. Figure 2a c shows the +3 charge state mass spectra of the seemingly (M/z=728.65180, Fig. 2a) And in theory (M/z=728.65172, Fig. 2b) Sulfated and the theoretically phosphorylated (M/z=728.65489, Fig. 2c) Tryptic proteins. since mass of (virtually any) Was much nearer to (n) rather than (f), We identified this adjustment as sulfation.
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